Diluting TriMix
Posted: Fri Dec 20, 2024 11:33 am
Over in this thread: https://www.franktalk.org/phpBB3/viewtopic.php?f=5&t=25512
I documented my start in the ICI world. A challenge I have is that my dose of 30/3/20 is hard to titrate because my ideal dose is somewhere between 1.25 and 3. It's very hard to measure the doses on my 0.3ml syringes - the gradient lines make it hard to be precise. In the other thread, I mused over the option of diluting the 30/3/20 to make measuring more repeatable.
I purchased two 30ml bottles of bacteriostatic water: one to empty to receive the newly combined solution and one to use for a water source. (I couldn't figure out how to procure an empty sterile bottle so emptying a bottle seemed the only way. Open to suggestions because it is very tedious to empty 30ml of water.) Then I put 1ml of TriMix and 3ml of water into the new bottle. I believe I now have a sterile, with benzyl alcohol for preservative, solution that is ΒΌ the strength of the original 30/3/20 TriMix. If I injected 2.5 units before, I will now inject 10 units. The goal being to more precisely inject 9 or 11 or whatever as my experiment continues.
Although I have made this new mix, I have a couple of days (the wife's in charge!) until I actually inject. Any comments or corrections or warnings would be appreciated.
I documented my start in the ICI world. A challenge I have is that my dose of 30/3/20 is hard to titrate because my ideal dose is somewhere between 1.25 and 3. It's very hard to measure the doses on my 0.3ml syringes - the gradient lines make it hard to be precise. In the other thread, I mused over the option of diluting the 30/3/20 to make measuring more repeatable.
I purchased two 30ml bottles of bacteriostatic water: one to empty to receive the newly combined solution and one to use for a water source. (I couldn't figure out how to procure an empty sterile bottle so emptying a bottle seemed the only way. Open to suggestions because it is very tedious to empty 30ml of water.) Then I put 1ml of TriMix and 3ml of water into the new bottle. I believe I now have a sterile, with benzyl alcohol for preservative, solution that is ΒΌ the strength of the original 30/3/20 TriMix. If I injected 2.5 units before, I will now inject 10 units. The goal being to more precisely inject 9 or 11 or whatever as my experiment continues.
Although I have made this new mix, I have a couple of days (the wife's in charge!) until I actually inject. Any comments or corrections or warnings would be appreciated.