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has anyone experienced that with a larger dose of trimix, it gets less hard?
My usual dose is 20 U trimix (since a year and a half) 30:1:10.
I experimented with 25 U trimix three times and each time it gets less hard compared with if I use the regular dose of 20 U (like 6 or 7 with 25 and 8 or 9 with the 20). The duration is the same with both higher and regular doses, not longer.
What might be the reason for this?

Hello! I have found that with the higher doses I get a harder erection for longer duration, without exception. Sometimes for 5 hours with the larger doses, so I only give a dose which lasts for 2 or 3 hours. I've never experienced an erection that was less than 1 hour.
Your experience may be coincidental, or related to where the injection was given and how well the trimix was absorbed and therefore metabolized.
Just keep trying and record your results. Next month I will have been taking trimix for 2 years now with excellent results!