Being relatively new to Trimix use, I experimented low to high, to find the dose, but keeping it frozen, with a couple minutes with the vile in your hand, to unfreeze (fill the syringe, then put it back in the freezer) , my initial dose and prescription worked just as well at 8 months, as it did at month 2.
A recent study performed by a well-known toxicologist and pharmacologist of some 14 common drugs from the 1960's showed that 12 of the 14 had zero degradation (identical performance), and the other two medications were still at 80+% of original benefit.
I can't remember if this stat is only that of the U.S., or worldwide, but apparently (and due to *use-by* labeling, some 800 million dollars worth of medications are thrown away yearly by pharmacies and hospitals yearly, which leave one wondering how our legislators and the FDA would require such waste, unless billions in profits are the reason, which this study seems to indicate. Just a heads-up, as apparently even the AMA tried to have these dates extended, yet met with push back.